PPARγ基因转录与单核巨噬细胞来源的泡沫细胞形成

PPARγ gene expression and macrophage-derived foam cell formation

目的 :探讨PPARγ基因转录在动脉粥样病变局部的作用。方法 :观察动脉粥样硬化局部PPARγ基因表达与巨噬细胞来源的泡沫细胞、清道夫受体A(SRA)分布的关系 ;分析PPARγ配体对氧化低密度脂蛋白 (ox LDL)介导的巨噬细胞SRA、PPARγmRNA表达、肿瘤坏死因子α(TNF α)和基质金属蛋白酶 9(MMP 9)释放的影响。结果 :PPARγ基因表达与巨噬细胞来源的泡沫细胞、清道夫受体A(SRA)的分布相似 ;PPARγ配体在增加巨噬细胞PPARγmRNA表达的同时 ,显著抑制了ox LDL介导的巨噬细胞SRAmRNA表达和TNF α、MMP 9的释放。结论

Objective:To probe the roles of PPARγ gene in macrophae\|derived foam cell formation Method:The relationship among PPARγ、Scavenger receptor A(SRA)gene expression and macrophage\|derived foam cells in atherosclerotic plaque of the New Zealand White rabbits were observed,and the effects of PPARγ ligand on SRA and PPARγ gene expression\,cytokines(TNF α and MMP 9)release induced by ox\|LDL in monocyte\|macrophages were analyzed.Result:(1)Most of the foam cells in the atherosclerotic lesions came from monocyte\|macrophage cells,PPARγ and SRA mRNA were expressed in both early and advanced atherosclerotic lesions,and which were located mainly in macrophage\|derived foam cells;(2)PPARγ ligand significantly increased macrophage PPARγ mRNA expression,and obviously decreased macrophage SRA mRNA expression\,TNF α and MMP 9 protein release induced by ox\|LDL.Conclusion:PPARγ gene activation may suppress SRA gene expression and the release of proinflammatory cytokines,PPARγ ligand may be helpful for lowering lipid contents in atheroma plaque.