P物质对培养成纤维细胞c-fos、c-jun表达的影响

Expression of c-fos and c-jun after the Stimulating Effects of Substance P on the Cultured Fibroblast

目的 研究P物质刺激体外培养成纤维细胞后转录因子c fos、c junmRNA及其蛋白在不同时相点表达的规律 ,探讨c fos、c jun在P物质调控创面愈合中的信号转导作用。方法 采用Wistar大鼠肉芽组织成纤维细胞体外培养模型 ,设立实验组和对照组 ,分别用原位杂交和SABC免疫组化方法检测P物质刺激后c fos、c junmRNA及其蛋白表达的变化规律及特征。结果 P物质刺激后c fos、c junmRNA及其蛋白表达增加 ,其中c fos、c junmRNA在刺激后 1h达峰值 ,Fos蛋白在刺激后 2h达峰值 ,Jun蛋白在刺激后 3h达峰值。结论 P物质可以上调c fos、c junmRNA及其蛋白的表达 ,提示原癌基因c fos、c

Objective To investigate the rule of the expressions in different time points of c-fos and c-jun mRNA and their proteins after the stimulating effects of substance P on fibroblast, so as to explore the transduction singals of c-fos and c-jun in prompting wound healing of substance P.Methods Cultured Wistar rat's granulatious fibroblasts were divided into substance P stimulating group and control group. In situ hybridization and immunohistochemical methods were used to evaluate the characteristics and pattern of the changes in the c-fos ,c-jun mRNA and their proteins, respectively.Results It was found that the expressions of c-fos ,c-jun mRNA and their proteins could be obviously increased after the stimulating effects of substance P. The expressions of c-fos and c-jun mRNA reached peak level at 1 postburn hour(PBH) and FOS protein at 2 PBH, JUN protein at 3 PBH.Conclusion The expressions of c-fos and c-jun could be increased after the stimulating effects of substance P, suggesting that c-fos and c-jun were probably one of the transcription factors in the regulating of wound healing.