摘要
目的建立赶黄草中槲皮素反相高效液相色谱法(RP-HPLC)含量测定方法。方法采用RP-HPLC对槲皮素进行定量测定;采用Kromasile C18柱(4.6 mm×150 mm,5μm)色谱柱,检测波长为256 nm。结果 色谱条件为:流动相:甲醇-0.2％磷酸溶液(55:50),柱温:25℃;流速:1.0mL·min-1。此法线性范围6.7～40.2μm·mL-1,r=0.9980,方法的精密度RSD为1.65％(n=5)。平均回收率为98.3％,RSD为1.73％(n=5)。结论 测定方法简便快捷、精密度高、准确性好,可以作为赶黄草中黄酮的测定方法,以控制其原料的质量。
OBJECTIVE: To establish a RP-HPLC method for the determination of quercetin in Penthorum chinense Pursh. METHODS: The content of quercetin was determinated by RP-HPLC. Kromasile C18 column (4.6 mm × 150 mm,5 μm) was used. The detection wave-length was set at 256 nm. RESULTS: The mobile phase consisted of methanol-0.2% phosphatic acid (55:50) with flow rate 1.0 mL·min-1 and column temperature at 25 °C. The method was liner within the range of 6.7 ∼ 40.2 μg·mL-1 (r = 0.9980). The method RSD was 1.65% (n = 5). The average recovery of assay was 98.3% and RSD was 1.73% (n = 5). CONCLUSION: The method appeared to be simple and rapid, highly accurate and precise. It can be used for the quantitative determination of quercetin in Penthorum chinense Pursh.
出处
《中国药学杂志》
EI
CAS
CSCD
北大核心
2004年第2期97-98,共2页
Chinese Pharmaceutical Journal
