草生欧文氏菌(E.herbicola)CSH1065质粒的重组标记及Fib基因的遗传转移

Genetic Recombination and Fib Genes Transfer of the Plasmid of E. herbicola CSH 1065

利用DNA分子克隆技术及遗传重组方法,在E.herbicola CSH1065质粒上插入了Km_R及Mob基因,利用细菌间的接合转移,使E.herbicola CSH1065质粒转移到E.coli HB101中,从而获得了E.herbicola CSH1065的Fib(fungi inhibition)基因在E.coli HB101中的表达,进一步证明E.herbicola CSH1065 Fib基因功能只涉及其细胞内的大质粒,与其染色体基因组无关,其黄色色素基因与Fib功能无关。这些结果还为DNA分子杂交方法所证实。

We inserted kanamycin resistance (Km) gene and mobilized function (Mob) gene on the plasmid of E. herbicola CSH1065 by using DNA molecular cloning and genetic recombination techniques. Therefore, the plasmid of E.herbicola CSH1065 to E. colt HB101 could be transferred by conjugation, the expression of those genes concerning fungi in-hibition function (Fib) of E. herbicola CSH1065 in E. colt HB101 was observed. This result confirmed again the fungi inhibition genes of E. herbicola CSH1065 is only related to its plasmid genome not to its chromosome genome. Meanwhile those yellow pigment genes located on the plasmid don't involve the antifungi function of E. herbicola CSH1065. All those results were convinced by DNA molecular hybridizations.