SOD-二茂铁修饰电极的电化学行为

Electrochemical behaviors of superoxidedismutase(SOD)-ferrocene modified gold electrode

Ferrocene and SOD-ferrocene modified gold electrodes were made by crosslinkage method with fress egg white and glutaraldehyde,and cyclic voltammetry was used to study these electrodes in phosphate buffer solution of different pH values and different concentrations of H2O2.The results indicated that in alkaline solution the cathodic response of ferroence was inhibited more greatly than the anodic response of ferrocene.The results also indicated that the cathodic peak current of ferrocene decreased rapidly while the anodic peak current decrased slightly with the addition of H2O2.But the ferrocene modified on the electrode was not lost,so we concluded that ferroence（+1）could be catalytic to the decomposition of H2O2.The behavior of SOD-ferrocene modified electrode indicated that SOD could prohibit the decrease of the peak current of ferrocene in the presence of H2O2.But with the increase of concentrations of H2O2 the peak currents of SOD-ferrocene modified electrode began to decrease too;and it could take on the same electrohehavior of ferrocene modified electrode when the concentrations of H2O2 are high enough.