间接免疫荧光法鉴别小鼠早期胚胎性别的研究

Identification of Sex in Mouse Preimplantation Embryos by Indirect Immunofluorescent Assay

以间接免疫荧光法检测昆明系小鼠早期胚胎雄性特异性H—Y抗原的表达。结果表明:54%的胚胎为H—Y阳性(雄性);46%为H—Y阴性(雌性)。经与昆明系小鼠的自然性比率(♂:52%;♀:48%)比较,两者无显著性差异。 经细胞遗传学方法确证,以免疫荧光法鉴别的胚胎性别,雄性的鉴别准确率为75.50%;雌性为82.97%。 本文还就影响鉴别准确率的若干因素以及本方法在生产中的应用前景等问题进行了分析讨论。

Indirect immunofluorescent assay was used for detecting male-specific H-Y antigen on preimplantation embryos. Our results showed that, embryos examined, 54.1% were H-Y positive (male) and 45.9% were H-Y negative (female). Significant difference was not found between natural sex ratio of kunming strain mouse (♂:52.1%; ♀:47.9%) and our results (P>0.05).Moreover, the results sequentially were confirmed by cytogenetic method. 75.50% H-Y positive embryos were male and 82.97% H-Y negative embryos were female respectively (P< 0.01).Some factors influencing the acouency of sex selection with indirect immunofluorescent assay were also discussed.