水稻野败型细胞质雄性不育系和其保持系蛋白质多肽的比较研究

Comparative Studies on the Constitutions of Protein Polypeptides Between a WA Type Cytoplasmic Male Sterile Rice and Its Maintainer

应用单向SDS—PAGE结合蛋白质铬银染色技术对水稻野败型细胞质雄性不育系珍汕97A和其保持系的叶绿体、线粒体和细胞质的蛋白质多肽进行了比较研究,发现两系之间存在明显的差异,生殖器官(穗子)上的差异比营养器官(叶片)上的差异更为显著。在成熟穗上,叶绿体可溶性蛋白不育系有25条带,保持系仅16条带,两者间有19个多肽不同;线粒体可溶性蛋白不育系有28条带,保持系比不育系少30.1和21.8KD两个多肽;细胞质可溶性蛋白丙酮沉淀物的水溶性蛋白组分不育系有24条带,保持系为29条带,两系间却有7条多肽存在差别;细胞质可溶性蛋白丙酮沉淀物的SDS-增溶性蛋白组分不育系有18条带,保持系只有11条带,两者间亦有7条多肽出现差异。由此可以看出,水稻野败型CMS表型的表达可能需要较多个基因的启动和关闭,既与叶绿体和线粒体有关,还涉及到核基因组的作用。

Comparative studies of the protein polypeptides of chloroplast, mitochondria and cytosol in the leaves or heads between the CMS rice Zhen Shan 97 A and its maintainer were performed. It was found by one dimentional SDS-PAGE and silver staining of proteins that differences of the proteins were more obvious in the organs of reproduction (heads) than in the organs of vegetation (leaves).In mature heads, the CMS rice had 14 bands of the soluble proteins of chloroplast, 2 bands of the soluble proteins of mitochondria, 1 band of the water-soluble compoments of the acetone precipitations of cytosol proteins and 5 bands of the SDS-aid-soluble components of the acetone precipitations of cytosol proteins which the fertile rice did not synthesize. The CMS rice, however, did not produce 5 bands of the soluble proteins of chloroplast, 6 bands of the water-soluble components of the acetone precipitations of cytosol proteins and 2 bands of the SDS-aid-so-iuble components of the acetone precipitations of cytosol proteins which the maintainer had. These abnormal phenomena in the synthesis of proteins in the CMS mature heads indicate that the expression of WA type CMS phenotype is initiated or closed by multi-genes, which is related to chloroplast, mitochondrial, and nuclear genome as well.The SDS-aid-soluble components of the acetone precipitations of cytosol proteins were variable at different developing stages of plants. Furthemore, the difference of these proteins between the CMS rice and its maintainer existed in both leaf and head cells before and after the pollen aboning stage. It is suggested that some polypeptides of these proteins may play a regulating role in the expression of WA type CMS of rice.