β1,4-半乳糖基转移酶-Ⅰ对施万细胞增殖的影响

STUDIES ON THE RELATIONS OF EXPRESSION OF β1,4- GALACTOSYLTRANSFERASE-Ⅰ AND PROLIFERATION OF SCHWANN CELLS

目的 探讨周围神经施万细胞表达 β1,4 半乳糖基转移酶 Ⅰ (β 1,4 GalT Ⅰ )对其增殖的影响。 方法 将构建的正、反义 β 1,4 GalT Ⅰ表达质粒 ,转染到施万细胞中 ,分析转染细胞倍增时间、3H TdR掺入情况以及细胞周期变化。 结果 转染正义β 1,4 GalT Ⅰ后施万细胞的增殖受到抑制 ,这种作用随转染浓度增大而增强 ;在G1 /G0 期的细胞数目增加 ,S期的数目减少。而转染反义 β 1,4 GalT Ⅰ有相反的作用。 结论 β 1,4 GalT Ⅰ对施万细胞的增殖有抑制作用 ,可能在周围神经施万细胞的增殖调节中发挥重要作用。

Objective In order to study the effect of β 1,4 GalT Ⅰ on proliferation of Schwann cells, The changes in cell proliferation were checked after Schwann cells transfected with sense or antisense β 1,4 GalT Ⅰ plasmids. Methods Methods of counting numbers of proliferating cell and measuring [ 3H] thymidine incorporation by liquid scintillation were used to study the proliferation of purified Schwann cells which transfected with β 1,4 GalT Ⅰ plasmids. Cell cycles of those transfected cells were determined by fluorescence activated cell sorting. Results After Schwann cells transfected with sense β 1,4 GalT Ⅰ plasmids, the proliferation of those cells was restrained, and the number at G 1/G 0 phase of those cells increased while the number at S phase decreased. Schwann cells transfected with antisense β 1,4 GalT Ⅰ plasmid showed opposite changes.Conclusion β 1,4 GalT Ⅰ may play an important role in regulating the proliferation of Schwann cells in peripheral nerve.\;[