帕金森病大鼠模型的改进及神经前体细胞移植治疗的行为学研究

An improved establishment of Parkinson's disease rat model and praxiology study on the Therapeutic transplantation of neural precursor cells

目的探讨提高 6 羟多巴胺 (6 OHDA )帕金森病大鼠模型成功率的技术改进方法 ,并对改进模型进行行为学评价。观察神经前体细胞定点移植对改进型PD大鼠模型行为学的影响。方法 6 O HDA双点微量注射于大鼠左侧大脑制备PD大鼠模型并观察其行为学变化。小鼠胚胎干细胞的培养和无血清神经诱导 ,神经前体细胞脑内移植 ,移植后PD大鼠行为变化。结果改进注射方法后PD大鼠模型制备成功率为 73 .3 %,较常规制备方法明显提高 ;神经前体细胞脑内移植后的PD大鼠的旋转次数明显减少 ;结论使用 6 OHDA双点注射选择性破坏大鼠多巴胺能神经元 ,可建立较稳定的且成功率较高的PD模型。小鼠ES细胞诱导的神经前体细胞脑内移植后PD大鼠旋转次数明显减少。

ObjectiveTo explore an improved method of 6-OHDA-lesioned rats model for Parkinson's disease and to evaluate the praxiology of the model. The behavioral influences of the fixed-point transplantation of neural precursor cells on the PD rats were also observed.Methods After stereotaxic microinjection of 6-hydroxydopamine(6-OHDA) into two points of the rat left brain, the changes in praxiology were observed, and the mouse embryonic stem cells were cultured and then induced into neural precursor cells with serum-free medium. After the intracerebral transplantation of neural precursor cells,the praxiology of the model were observed again.Resultsthe success rate of rats model rises to 73.3% by improving the injection method, which is much better than that of the usual procedures;and the rotation rate of the PD rats is obviously decreased after the intracerebral transplantation of neural precursor cells. ConclusionsAn Parkinson's disease rat model was established steadly with high success rate, while slective lesion of dopaminergic neurons could be performed by using the two points 6-OHDA-microinjection. And when the mouse embryonic stem cells are induced and differentiated into neural precursor cells in vitro, the latter one could transplant into brain, which leads to the obvious decrease of rotation rate in rats model.