微波辐射致大鼠海马损伤中MAPK传导通路的差别激活(英文)

Differentiate activation of MAPK conductive gateway in hippocampus damages in duced by microwave irradiation in rat

目的:探讨微波辐射对海马神经神经细胞有丝分裂素激活蛋白激酶类(mitogen-activatedproteinkinases,MAPK)信号转导系统的影响,以及MAPK信号转导通路在微波辐射导致海马神经损伤中的作用。方法:采用抗磷酸化形式的细胞外调节蛋白激酶(extracellularsynal-regulatedkinase,ERK1/2),C-Jun氨基末端激酶(C-JunN-terminalkinase,JNK),P38MAPK抗体,进行Westernblot,动态观察微波辐照后大鼠海马神经细胞ERK,JNK/SAPK,P38MAPK蛋白质磷酸化情况,以此反映MAPK信号通路的功能状态。结果:微波辐照后12h内ERK,JNK持续激活,ERK的激活高峰在辐照后即刻,JNK激活高峰出现于辐照后3,24h后两者活化均受抑制;P38MAPK辐射后24h内一直处于激活,并在3,24h出现两次激活高峰。结论:微波辐射可使海马神经细胞3条MAPK信号转导通路出现差别激活,并且微波辐射导致的海马神经损伤可能是通过MAPK信号转导通路的差别激活实现的。

AIM:To discuss the effects of microwave irradiation on the neurocyte mitrogen-activated protein kinases(MAPK) signal conductive system in hippocampus nerve and the reaction of MAPK conductive gateway in hippocampus neural damages. METHODS:Anti-phosphorylation extracellular synal-regualted kinase(ERK1/2) ,C-Jun N-terminal kinase(JNK) and P38 MAPK antibody were introduced in Weste rn blot process to dynamically observe the phosphorylation changes of rat hippoc ampus neurocyte protein ERK,JNK/SAPK and P38 MAPK in order to reflect the functi onal status of MAPK signal gateway after microwave irradiation. RESULTS:Within 12 hours after microwave irradiation,ERK and JNK were continuo usly activated,the peak of ERK appeared immediately after irradiation,and JNK pe ak appeared after 3 hours of irradiation.And after 24 hours,both activations wer e inhibited;P38 MAPK was continuously in activating status within 24 hours after irradiation,and there were 2 activating peak appeared at 3 and 24 hours after i rradiation. CONCLUSION:Microwave irradiation can induce differentiate activation in three MAPK signal conductive gateways of neurocyte of hippocampus and moreover,the hi ppocampus damages induced by microwave irradiation might be achieved through the differentiate activation of MAPK signal conductive gateway.