转化生长因子-β_1诱导自体骨髓间质干细胞移植修复兔耳郭软骨缺损

An experimental study on autologous bone marrow mesenchymal stem cells induced with transforming growth factor β_1 for the repair of defect of auricular cartilage in rabbits

目的:探讨转化生长因子-β1(TGF-β1)诱导自体骨髓间质干细胞(MSCs)修复兔耳郭软骨缺损的可行性。方法:取新西兰大白兔MSCs体外培养扩增,以含TGF-β1、地塞米松和维生素C的培养液做诱导培养,以诱导后的软骨细胞和高孔隙率聚乳酸(PDLLA)形成复合物为实验组修复兔自体耳郭软骨缺损,对照组仅进行单纯PDLLA移植,分别于移植后6,12,18周取材,行苏木精-伊红和甲苯胺蓝染色,观察各组兔耳郭软骨缺损修复情况。结果:术后18周缺损区完全由软骨组织修复,缺损表面光滑,软骨陷窝明显,与周围正常软骨组织无明显界限,单纯PDLLA对照组为纤维软骨和纤维组织修复。结论:TGF-β1诱导自体骨髓间质干细胞可用于修复兔耳郭软骨缺损。

AIM:To investigate the feasibility of autologous bone marrow mesenchymal stem cells(MSCs) induced by transforming growth factor(TGF β1) for the repair of rabbit auricular cartilage defects. METHODS:Proliferation of MSCs of New Zealand rabbits were cultured in vitro.The culture medium composed of TGF β1,dexamethasone,and ascorbic acid was induced to culture the SMCs.The tissue engineered cartilaginous graft was composed of chondrogenetic MSCs/poly D,L lactic acid(PDLLA) and then implanted into the defect of autologous auricular cartilage in rabbits as trial group.The PDLLA without cells was served as control group.Samples were taken out at 6,12 and 18 weeks after implantation,and then were stained with hematoxylin and eosin,and toluidine blue to observe the repair of rabbit auricular cartilage defects. RESULTS:The MSCs could repair the rabbit auricular cartilage defects with cartilage tissue completely after 18 weeks of the transplantation,and smooth defect surface and obvious cartilage lacuna were observed,which were no significant difference from the normal peripheral cartilage tissue,but the control group was repaired with fibrous cartilage or fibrous tissue. CONCLUSION:The induced autologous MSCs derived from bone marrow can repair the rabbitauricular cartilage defects.