缺血再灌注后不同时间正常肝组织和肿瘤组织超氧化物歧化酶和丙二醛含量变化(英文)

Changes in the contents of superoxide dismutase and malondialdehyde in normal liver and tumor tissue after ischemic reperfusion of hepatic neoplasm in different periods

背景:缺血再灌注对正常组织具有损伤作用,但是它对于肿瘤组织的影响以及在肿瘤患者术后康复中的作用还不清楚。目的:探讨缺血再灌注对肿瘤组织的影响及其在肿瘤患者术后康复中的意义。设计:采用完全随机对照研究。地点和对象:实验在第四军医大学唐都医院实验外科进行,参加人员为本科全体医护人员。实验动物为第四军医大学动物实验中心提供的36只成年新西兰白兔(雌雄不拘)。干预:通过超声引导将VX2肿瘤组织混悬液穿刺注射到新西兰兔肝脏左中叶,建立肝脏肿瘤模型,用无损伤血管钳阻断肿瘤所在肝叶的肝动脉分支60min后去除血管阻断恢复血流,造成肝脏肿瘤的缺血再灌注损伤模型。主要观察指标:各组实验动物的肝脏组织和肿瘤组织的超氧化物歧化酶(SOD)和丙二醛(MDA)的含量。结果:肝脏组织中的SOD含量于缺血再灌注后迅速下降,至0min达最低点,随后有所升高,至7d时仍明显低于缺血再灌注前水平,各组与对照组对比差异显著(t=24.83～65.01,P<0.01),而肿瘤组织的SOD含量变化趋势除了1h达最低点外其余皆与肝脏组织相似,各组与对照组对比差异显著(t=4.68～12.42,P<0.01);肝脏组织的MDA含量于0min时升至最高,随后开始下降,至7d时仍高于缺血再灌注前水平,各组与对照组对比差异显著(t=7.39～42.05,P<0.01),而肿瘤组织的MDA?

BACKGROUND:Ischemic reperfusion plays an injurious role in the normal tissue and organ, but its influence and implication on the hepatic neoplasm and its implication to the rehabilitation of the postoperative neoplasm patients are not clear. OBJECTIVE:To explore the influence of the ischemia reperfusion on the hepatic neoplasm and its complication to the rehabilitation of the postoperative neoplasm patients. DESIGN:Completely randomized design of multiple groups was taken, the control group was built, and the contrasts of the groups with the control group were made respectively. SETTING AND PARTICIPANTS:The experiments were performed in the department of experimental surgery, Tangdu Hospital of Fourth Military Medical University by the faculty of the department. The 36 white New Zealand adult rabbits (male or female) were collected from the experimental animal centre of Fourth Military Medical University. INTERVENTION:The animal model of hepatic neoplasm was set up by the ultrasonography guided implantation of VX2 tumor constitution mass into the left middle lobe of liver of rabbits. Ischemia was caused by using a non traumatic vascular clamp to block the branches distributing in the left middle lobe of the hepatic artery for 60 minutes, and then the clamp was removed and the reperfusion injury of hepatic neoplasm occurred. MAIN OUTCOME MEASURE:The concentrations of super oxide dismutase(SOD) and malondialdehyde(MDA) of the normal hepatic tissues and the neoplasm tissues from the livers of all the experimental animals were detected. RESULTS:The SOD concentration of hepatic tissue decreased quickly to the lowest point at 0 minutes after ischemia reperfusion and then restored gradually, but still retained a lower level than that of the control group at 7 days(t= 24.83-65.01,P< 0.01 vs the control group). The variation of the SOD concentration of neoplasm tissue was similar to that of hepatic tissue except that in the 1 hours group which reached the lowest point (t=4.68-12.42,P< 0.01 vs the control group). The MDA concentration of hepatic tissue increased to the highest point at 0 minutes and then dropped, but still retained a higher level than that of the control group at 7 days(t= 7.39-42.05,P< 0.01 vs the control group). The MDA concentration of neoplasm tissue decreased to the lowest level at 1 hours and restored gradually, but still retained a lower level than that of the control group at 7 days(t= 8.65-18.07,P< 0.01 vs the control group). CONCLUSION:The production and injurious effect of SOD of neoplasm tissue are more serious than those of the normal hepatic tissue, which perhaps plays a positive role in the rehabilitation of the post operative neoplasm patients.