甘草酸对人乳腺癌细胞增殖抑制和诱导凋亡作用的研究

Proliferative Inhibition and Apoptotic Induction Effect of Glycyrrhizin on Human Breast Carcinoma Cells

目的 探讨甘草酸对人乳腺癌细胞 (MCF 7)增殖抑制和诱导凋亡的作用 ,并探讨凋亡发生与细胞内Ca2 + 浓度( [Ca2 + ]i)变化的关系。方法 2 .5～ 12 .5mmol/L甘草酸处理MCF 7细胞 2 4h ,采用MTT比色法测定细胞增殖能力。 5 .0mmol/L、7.5mmol/L和 10 .0mmol/L甘草酸处理MCF 7细胞 2 4h ,用末端脱氧核苷转移酶介导dUTP末端标记法和AnnexinV流式细胞仪法检测凋亡细胞。 7.5mmol/L甘草酸处理MCF 7细胞 2 4h ,采用Fura 2荧光负载方法测定 [Ca2 + ]i的变化。结果 甘草酸从 5 .0mmol/L浓度起对MCF 7细胞的增殖抑制率显著升高 (P <0 .0 1) ,呈剂量依赖性 ;半增殖抑制浓度 (IC50 )为 15 .8mmol/L。 7.5mmol/L和 10 .0mmol/L甘草酸使细胞凋亡率显著升高 (P <0 .0 5和P <0 .0 1)。甘草酸处理组的 [Ca2 + ]i明显低于对照组 (P <0 .0 5 )。结论 甘草酸具有抑制MCF 7细胞增殖和诱导凋亡的作用 ;其诱导细胞凋亡可能与细胞内Ca2 +

Objective To investigate the proliferative inhibition and apoptotic induction effect of glycyrrhizin(GL) on human breast carcinoma(MCF-7) cells.The relationship between GL-induced apoptosis effect and intracellular Ca 2+ concentration was also studied.Methods After MCF-7 cells were treated with different concentration of GL from 2.5 mmol/L to 12.5 mmol/L for 24 h,cell viability of proliferation was assessed by MTT assay.MCF-7 cells were treated with 5.0 mmol/L,7.5 mmol/L and 10.0 mmol/L GL for 24 h,and the rate of cell apoptosis was examined by terminal deoxynucleotide transferase mediated dUTP nick-end-labeling method and flow cytometry with Annexin V/propidium iodide fluorescent stain.7.5 mmol/L GL was used for 24 h and intracellular Ca 2+ concentration was measured by Fure-2 fluorescein load method.Results With the concentration increase of GL from 5mmol/L to 12.5 mmol/L,the rate of proliferative inhibition increased significantly(P<0.01).The degree of the inhibition were in a dose-dependent fashion.IC 50 of the proliferative inhibition was 15.8 mmol/L.With GL of 7.5 mmol/L and 10.0 mmol/L,the rate of cell apoptosis increased significantly(P<0.05 and P<0.01).Intracellular Ca 2+ concentration was lower evidently when treated with GL(P<0.05).Conclusion Glycyrrhizin has the proliferation inhibition and apoptosis induction effect on MCF-7 cell.The descent of intracellular Ca 2+ level may be related to apoptosis induction effect of GL in MCF-7 cells.