摘要
目的:探讨清瘀胶囊中大黄素含量的测定方法,以控制该制剂的质量。方法:以C_(18)化学键合硅胶柱为固定相,甲醇-0.2 mol/L磷酸二氢钠溶液(磷酸调pH为3.0)(80:20)为流动相,检测波长为289 nm。结果:大黄素进样量在0.020～0.120μg范围内与峰面积值线性关系良好,r=0.9997,加样回收率为95.19％～98.03％。结论:反相高效液相色谱法测定结果准确,重复性好,可用于测定清瘀胶囊中大黄素的含量。
Objective: To establish a RP-HPLC method for determination of the content of emodin in Qingyu Capsule, so as to control the quality of this preparation. Methods: The column was C18, and the mobile phase was methanol-0. 2 mol/L NaH2PO4 solution (pH adjusted to 3.0 with H3PO4) (80:20). The detection wavelength was at 289 nm. Results: There was a good linear relationship between the concentration of emodin and absorption area value in the range of 0.020-0.120 μg( r=0.999 7) . The average recovery was 95.19%-98.03%. Conclusion: The method was highly repeatable, accurate, and can be applied to the quality control of Qingyu Capsule.
出处
《中国药业》
CAS
2004年第2期43-44,共2页
China Pharmaceuticals
