苏云金杆菌33菌株最佳培养基和发酵条件研究

Studies on Fermentation Excellent Culture Medium and Parameter for Bt33

应用正交试验L2 7(313)对鳞翅目昆虫高毒效的苏云金杆菌 33菌株的发酵培养基进行研究 ,得到了适合其发酵培养的优化复合培养基配方——— 0 .5 %玉米粉 +2 .0 0 %黄豆粉 +0 .15 %酵母粉 +0 .2 5 %鱼粉 +0 .0 75 %蛋白胨 +0 .2 5 %磷酸二氢钾 +0 .0 5 %碳酸钙 +0 .0 35 %硫酸镁。另外实验还证实适当增加通气量、发酵初始酸碱度控制在pH7.5、发酵温度 30℃、转速 180r/min、培养 4 0h更适合该菌株液体深层发酵。

The cultural medium of Bacillus thuringiersis 33 which is high poison to lepidoptera insect was researched by the orthogonal test.The results indicated that under the cultural condition of temperature 30℃,50ml inventory/500ml flask and speed of agitation 180r/min, cultivate 40 hours,the optium fermentation medium (%)was obtained which was composed of carbon source 0.5,corn flour 2.0,soybean flour 0.15,yeast powder 0.25,peptone 0.075,potassium dihydrogen phosphate 0.25,calcium carbonate 0.05,magnesium sulphate 0.035,pH7.5.