摘要
生物传感器是一种新兴的测试工具,其中酶电极因具有较常规离子选择电极更短的响应时间,更好的选择性与抗干扰能力强等特性,引起了人们的关注。
A catalase membrane immobilized on a nylon net was prepared, using freshly synthesized triethylonium tetrafluoroborate as immobilizer. The membrane had an activity of 80unit/cm^2,while that immobilized by dimethyl sulfate showed an activity only of 35, 6unit/cm^2 .The catalase electrode showed linear response within 5, 0×10^(-6)～5,0×10^(-4)mol·L^(-1),with detection lirit of 4,0×10^(-6)mol·L^(-1) and 95,2% recovery.Its service life is more than 500 times. SO_4^(2-), Cl^-, NO_3^-,NH_4^+,Ac^-,glycine,glueose etc. do not interfere with the detection.
出处
《应用化学》
CAS
CSCD
北大核心
1992年第1期89-91,共3页
Chinese Journal of Applied Chemistry
关键词
过氧化氢酶
酶电极
固定化
H2O2
catalase, enzyme electorde, immobilization, hydrogenperoxide determination
