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A型流感病毒基质蛋白研究进展 被引量：2

The Research Progress on lnfluenza A virus Metrix Protein
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摘要 流感病毒基质蛋白 MA包括 M1和 M2两种 ,在病毒进化过程中其结构是稳定的。 A型流感病毒 M1在病毒复制及感染中起关键作用 ,而 M2是一种跨膜蛋白 ,结构很保守。 M2可调节高尔基体跨膜转移通道的 p H梯度 ,对 HA蛋白起稳定性作用。将 M2与乙肝病毒制成融合蛋白 ( M2 HBc)分别免疫小鼠 ,小鼠获得了 90 %～10 0 %的免疫保护力 ,且具有交叉性和持久性。用研制的含 M2基因的 DNA疫苗 ,免疫小鼠产生了 M蛋白特异性的抗体和 CTL反应 。 The MA of influenza A virus, including M1 and M2,has a stable structure during the evolution of virus.The M1 play a great role in the replication and infection of virus. M2,being a integral membrane protein, is highly conserved in amino acid. M2 regulates transmembrane pH gradients in the trans Golgi. These data provide direct evidence that the influenza virus M2 protein ion channel activity can affect the status of the conformational form of cleaved HA during intracellular transport. Intraperitoneal or intranasal administration of purified M2HBc particles to mice provided 90%-100% protection against a lethal virus challenge,which is broad-spectrum, long-lasting protection against influenza A infections.The present study also revealed that administering DNA vaccine by topical application can elicit both humoral and cell-mediated immunity (CMI).
作者 朱建国
机构地区 上海交通大学动物生物技术系
出处 《动物医学进展》 CSCD 2004年第2期24-26,共3页 Progress In Veterinary Medicine
关键词 A型流感病毒 基质蛋白 结构稳定性 生物学功能 免疫功能 免疫机制 核酸疫苗 Influenza A virus MA protein conserved structure
分类号 S852.659.5 [农业科学—基础兽医学]
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参考文献17

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同被引文献29

  • 1李明峰,程军,何志勇,熊伟民,吴祥甫.基因重组幽门螺杆菌HspA—Zot融合蛋白质口服疫苗的研制[J].中华腹部疾病杂志,2003,3(8):547-551. 被引量：1
  • 2郑丽舒,段招军.A型流感病毒M2蛋白疫苗的研究进展[J].病毒学报,2006,22(6):488-491. 被引量：1
  • 3姜建波,提金凤,李志杰.新型疫苗研究概况[J].动物医学进展,2007,28(1):96-100. 被引量：4
  • 4Palese P.Influenza:old and new threats[J].Nat Med,2004,12(S):82-87.
  • 5Horimoto T,Kawaoka Y.Influenza:lessons from past pandemics,warnings from current incidents[J].Nat Rev Microbiol,2005,3(8):591-600.
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  • 7Epidemic and Pandemic Alert and Response (EPR).Cumulative Number of Confirmed Human Cases of Avian Influenza A(H5N1) Reported to WHO[EB/02].Http://www.who.int/csr/disease/avian-influenza/country/cases-table-2007-04-12/en/index.html.
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  • 10Duong-Ly K C,Nanda V,Degrado W F,et al.The conformation of the pore region of the M2 proton channel dependson lipid bilayer environment[J].Protein Sci,2005,14(4):856-861.

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动物医学进展
2004年 第2期

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