阿奇霉素对弓形虫病大鼠肝细胞周期和细胞凋亡及NO水平的影响

Effects of Azithromycin on Apoptosis and Cell Cycle of Liver Cells and Notevl in Rats Infected with Toxoplasma gondii

为了研究阿奇霉素对弓形虫病大鼠肝细胞周期及细胞凋亡及血清 NO水平的影响。用3 0只成年雄性 SD大鼠 ,随机分为感染组、治疗组和空白对照组 ,每组各 10只。感染组每只腹腔注射 2× 10 5/ m L 活的纯化弓形虫速殖子悬液 2m L。治疗组每只腹注同上剂量纯化弓形虫速殖子悬液后 ,每只每天喂服阿奇霉素 2 0 0 m g/ kg体重治疗 ,连用 7d。空白对照组每只腹注生理盐水 2 m L。 9周后解剖所有的大鼠 ,取肝组织制成单细胞悬液 ,应用流式细胞仪检测其细胞周期及细胞凋亡率。同时应用硝酸还原酶法测定各组大鼠血清 NO水平。结果表明 :感染组大鼠肝细胞凋亡率 ( 10 .3 0± 2 .62 ) %显著高于空白对照组大鼠肝细胞凋亡率 ( 9.2 3± 3 .0 3 ) % ( P<0 .0 5 )。治疗组大鼠肝细胞凋亡率 ( 9.73±3 .73 ) %明显低于感染组大鼠肝细胞凋亡率( 10 .3 0± 2 .62 ) % ( P <0 .0 5 )。治疗组大鼠肝细胞凋亡率与空白对照组大鼠肝细胞凋亡率相比较 ,无显著性差异 ( P >0 .0 5 )。肝细胞周期中 S期肝细胞比例感染组较治疗组低 ( P <0 .0 5 ) ,而 G1 期比例二者相反。治疗组与空白对照组比较细胞周期无显著性差异 ( P >0 .0 5 )。同时 ,感染组大鼠血清 NO平均水平为 ( 10 3 .2 6± 3 7.14 )μm ol/ L,治疗组大鼠血清 NO平均为 (

The objective of the study is to detect the effects of azithromycin on apoptosis and cell cycle of liver cells and NO level of sera in rats infected with Toxoplasma. 30 Sprague-Dawley rats were divided into three groups: infected group,treated group and control group with 10 rats for each group. The infected group rats were ip. Injected by 2×10~5/mL KS live Toxoplasma tachyzoites 2 mL. Treated group rats were treated by 200 mg/kg of azithromycin for 7 d after second with injection.Control group rats were ip. Injected with 0.9% NaCl_2 mL. After nine weeks, all of the rats were killed to obtain liver cells. The liver cell's apoptosis and cell cycle were detected by flow cytomety (FCM), while the level of NO in sera were detected by means of nitric acid reducase. The results showed: apoptois rate of liver cells in the infection group(10.30±2.62)% was obviously higher than that in control group(9.23±3.03)%( P <0.05). The apoptosis rate of liver cells in treatment group(9.73±3.73)% was remarkably lower than that in the infection group( P <0.05). The apoptosis rate in the treatment and the control group showed no significant difference. The S cycle of liver cell cycle in infected group was significantly less than that in treated group ( P <0.05), but the G1 stage proportion in infected group was significantly higher than that in treated group.The cell cycle in the treatment and the control group was no significant difference( P >0.05). The concentration of NO in sera were (103.26±37.14) μmol/L,((85.92)±42.13) μmol/L,and (81.03±21.46) μmol/L in infected group,treated group and control group,respectively.The level of NO in the infection group and treatment group were higher than that in the control group( P <0.05), but that in the treatment group was lower than that in the infection group respectively ( P <0.05).