猪伪狂犬病病毒新W株的分离与鉴定

Pseudorabies virus Isolated and Indentified of Xinjiang

从新疆五家渠、石河子某猪场病死仔猪的大脑和内脏组织中分离到一株疑为猪伪狂犬病病毒 ( PRV)毒株 ,通过兔体接种、BHK-2 1细胞培养、理化特性测定、中和试验、实验兔和仔猪回归试验、电镜观察、PCR扩增及感染力测定 ,分离的病毒接种实验兔后发生奇痒并麻痹致死 ;BHK-2 1细胞培养盲传 4代出现典型细胞病变 ;电镜观察可见病毒粒子呈圆形并带有囊膜和纤突 ;回归兔出现典型奇痒症状 ,仔猪出现典型的症状和病理变化 ;分离的病毒能被 PR鄂 A株标准阳性血清中和 ;该病毒对氯仿、乙醚、酸、热敏感 ;PCR体外扩增可见其与鄂 A株在同一水平位置上有相同的电泳带 ;用 BHK-2 1细胞测定其毒价 TCID50 为 10 -1 0 .87/0 .1m L 根据此分离病毒株的上述特性 。

One Pseudorabies(Aujeszky's disease) virus isolated from infected domestic pigs originating from Wujiaqu shihezi of ×injiang.The organ samples were filtered then drained monolayers of BHK-21 cells,the typical cytopathic effect(cpe) were observed. The virus appeared typical Pseudorabies virus form:diameter was about 100 nm,circle with peplos and peplomer .The virus were neutralized with standard positive porcine sera using serum neutralization test(SNT). TCID_(50) of virus was 10-10.87/0.1mL. Rabbits showed typical syndrome of PR when they were inoculated with cells virus. PCR technique was applied for the detection PRV in cells and Amplified a fragment with desired size of 217 bp.According with virus characterisation from infected pigs,the virus was indentified as PRV.