复方红芪减方对周围神经再生影响的实验观察

Experimental observation of the effect of fufang hongqi jianfang on regeneration of peripheral nerves

目的:在前期实验已经证实复方红芪提取液局部应用对周围神经再生有效基础上,对该方进行减方后全身给药的研究。方法:30只SD雄性大鼠,体质量200～250g,各鼠编号后随机分为3组,钳夹损伤双侧坐骨神经,N组为对照组,每日灌服生理盐水,Q组每日灌服复方红芪提取液2mL,S组每日灌服减方提取液2mL,同条件下饲养6周,观察坐骨神经功能指数及单位视野有髓神经纤维计数。结果:坐骨神经功能指数红芪组(-30.2700±10.8001)和减方组(-19.1105±9.1048)与对照组差异无显著性意义(P>0.05);而红芪组与减方组比差异有显著性意义,且减方组优于红芪组(P=0.03)。有髓神经纤维计数复方红芪组(112.33±17.99)个/视野优于对照组(75.77±14.36),(P=0.000),减方组(109.26±15.73)个/视野明显优于对照组(P<0.01),而红芪组与减方组间差异无显著性意义。结论:复方红芪减方提取液可以促进周围神经再生,药效更为专一,与原方比无明显差异。

AIM:To study the general administration of fufang hongqi jianfang on the basis that extract of fufang hongqi fang is effective on the regeneration of peripheral nerves.METHODS:Thirty male SD rats were randomly divided into control group(group N),fufang hongqi fang group(group Q) and fufang hongqi jianfan group(group S) following bilateral sciatic nerves were injured by clamp. The rats in the three groups were perfused with normal saline,2 mL extract from fufang hongqi fang and 2 mL extract from fufang hongqi jianfang respectively every day,and then were fed under the same condition for 6 weeks to observe the functional indices of sciatic nerves and the count of myelinated nerve fibers per visual field.RESULTS:The functional indices of sciatic nerves were higher in the group S(-19.110 5±9.104 8) than in the group Q(-30.270 0±10.800 1),showing significant difference between the two groups(P=0.03),while there was no significance between the control group and the group Q or group S(P >0.05).The number of myelinated nerve fibers was(112.33±17.99) per visual field in the group G and(109.26±15.73) per visual field in the group S,both higher than in the control group[(75.77±14.36) per visual field](P=0.000),but there was no significant difference between the groups G and S. CONCLUSION:Fufang hongqi jianfang can facilitate the regeneration of peripheral nerves,but has no significant difference with fufang hongqi fang on the curative efficiency.