电针损伤大鼠中脑后神经元结构和功能的变化(英文)

Structural and functional changes of midbrain neurons in rats injured by electroacupuncture

背景:神经元的功能状态与机体的生命活动密切相关。目前已有多种实验方法来鉴定神经元轴突的结构和功能变化。但对于树突,人们给予的重视还很少。目的:观察电针损伤大鼠中脑后神经元微管相关蛋白2(MAP2)免疫反应变化。设计:设立对照的动物实验研究。地点和材料:地点为吉林大学中日联谊医院中心实验室。Wistar大鼠,雌雄不限,体质量(180±120)g,每组6只动物。干预:电针损伤大鼠中脑,不同时间取脑组织,行MAP2免疫组织化学染色。主要观察指标:神经元MAP2免疫反应及不同时间病理变化。结果:正常大鼠脑组织MAP2免疫反应发生在神经元的树突和胞体。电针损伤后可见MAP2免疫反应不同的神经元:固缩神经元,轻度受损神经元,正常神经元。结论:在中枢神经系统损伤实验性研究中,MAP2免疫反应是观察神经元功能状态的一项好指标。

BACKGROUND:The function of neurons is closely relative with the health of human bodies.Presently,many methods have been used to detect the structural and functional changes of neuronal axons.But studies on dendrite are very limited.OBJECTIVE:To detect the changes of microtubule-associated protein-2(MAP2) immunoreaction in the midbrain of rats injured by electroacupunture.DESIGN:A case-control animal experiment. SETTING and MATERIALS:The experiment was made in the Medical Research Center,China-Japan Union Hospital of Jilin University.Wistar rats,male or female, weighing(180±120) g were subjected in the experiments.Each group included 6 rats.INTERVENTION:Brain tissues were taken out at different time to detect the changes of MAP2 immunoreaction, ollowing the midbrains of rat were injured with electroacupuncture. MAIN OUTCOME MEASURES:The changes of neuronal MAP2 immunoreaction and pathological changes at different time.RESULTS:Normal neuronal MAP2 staining was located mainly in the cytoplasm and dendrites.But after the injury of electroacupuncture,MAP2 immunoreactions were detected in different neurons: pycnosis neurons,gently injured neurons and normal neurons.CONCLUSION:MAP2 immunoreaction is a good marker to detect the function of neurons in the study of central nervous system injury.