盐度、碱度对中国林蛙蝌蚪及变态幼蛙的毒性影响

Effects of Salinity and Alkalinity on Tadpole and Abnormal Young Frog of Rana chensinensis

在水温 16～ 18℃的野外条件下 ,采用单因子静态急性毒性实验法 ,研究水环境中盐度、碱度对中国林蛙(Ranachensinensis)蝌蚪及变态幼蛙的毒性效应。在pH为 7 0～ 8 5、总碱度为 1 4 1mmol L时 ,盐度对中国林蛙蝌蚪及变态幼蛙的 2 4、4 8、72、96h半致死值 (LC50 )分别为 8 2 1、7 2 5、5 17和 3 70g L及 8 84、7 17、6 6 2、6 2 9g L ;零致死值(LC0 )分别为 7 14、6 0 0、2 6 7、2 2 0g L及 6 34、6 16、5 78、5 4 5g L ;全致死值 (LC1 0 0 )分别为 9 98、9 0 0、7 6 7、5 2 0g L及11 34、9 6 7、7 4 5、5 4 5g L ;安全值 (SC)为 1 70g L和 1 4 2g L。在相同pH下 ,盐度为 0 18g L时 ,碱度对中国林蛙蝌蚪及变态幼蛙的 2 4、4 8、72、96hLC50 值分别为 14 36、11 83、10 35和 7 6 8mmol L及 15 0 7、13 72、11 2 3、8 6 5mmol L ;LC0 值分别为 8 76、8 5 1、4 6 5、3 88mmol L及 9 11、7 0 3、6 85、5 17mmol L ;LC1 0 0 值分别为 19 96、15 14、16 0 5、11 4 8mmol L及2 1 0 2、2 0 4 1、15 6 0、12 0 2m mol L ;SC值为 2 4 1mmol L和 3 4 1mmol L。综合我国淡水养殖水质标准 ,建议养殖中国林蛙蝌蚪及变态幼蛙长期生存的盐度上限分别为 2 0g L和 1 5g L ,碱度上限分别为 2

Single-factor acute toxicity test was used to study the effect of salinity and alkalinity on tadpole of Rana chensinensis and abnormal young frog under the condition of water temperature between 16℃ and 18℃ outdoors. When water pH are 7.0～8.5 and alkalinity is 1.41、mmolL, the LC50 of the salinity at 24, 48, 72 and 96 hours to tadpole and abnormal young frog are 8.21, 7.25, 5.17, 3.70、gL and 8.84, 7.17, 6.62, 6.29 gL, respectively; the LC 0 are 7.14, 6.00, 2.67, 2.20 gL and 6.34, 6.16, 5.78, 5.45 gL, respectively; the LC 100 are 9.98, 9.00, 7.67, 5.20 gL and 11.34, 9.67,7.45, 5.45 gL, respectively; the SC are 1.70 and 1.42 gL, respectively. Under the same pH and salinity of 0.18 gL, the LC 50 of the alkalinity at 24, 48, 72 and 96 hours to tadpole and abnormal young frog are 14.36, 11.83, 10.35, 7.68 m molL and 15.07, 13.72, 11.23, 8.65 mmolL, respectively; the LC 0 are 8.76, 8.51, 4.65, 3.88 m molL and 9.11, 7.03, 6.85, 5.17 mmolL, respectively; the LC 100 are 19.96, 15.14, 16.05, 11.48 mmolL and 21.02, 20.41, 15.60, 12.02 mmolL, respectively; the SC are 2.41 and 3.41 mmolL, respectively. Combined with the standard of water quality for breed aquatics in fresh water, we suggested that the upper limit of salinity for tadpole and abnormal young frog to survive for a long time are below 2.0 and 1.5 mmolL, respectively; the upper limit of alkalinity are 2.5 and 3.5 mmolL, respectively.