间接荧光抗体法快速检测病禽组织中的新城疫抗原

Define Fast ND ANTIGEN IN Disease Poultry with IFA

利用间接荧光抗体法IFA对用新城疫病毒NDV攻毒鸡胚的卵黄、胚体、尿囊液、尿囊膜和人工感染鸡、自然病死鸡的心、肝、脾、肺、肾、脑、盲肠扁桃体、法氏囊等8种脏器进行检测。结果鸡胚收集液、6例人工感染和15例自然病例的脏器检测结果均为阳性;脏器以肝、脾、肺、脑的检出率最高,检出效果最好。

To examine chicken embry and organ that die of NDV.When the antigen was found in golk、embry、allantoic fluid、allantoic membrane、heart、liver、spleen、lungs、kidney、brain、cecal tonsil、Bursa of Fabricius etc in the same birds.The results showed that:All are positive.Liver、Spleen、lungs、Brain is the best organ and the best effect in defining.