丝胶-胰岛素结合物的制备及其生物特性研究

Preparation of Sericin-Insulin Bioconjugate and Its Biological Properties

以天然丝蛋白为原料,应用高温高压水解法和酸降解法制备分子量范围1～7万的水溶性丝胶蛋白粉末,以戊二醛为交联剂,与注射用胰岛素交联成丝胶-胰岛素生物结合物。应用酶联免疫吸附试验(ELISA)对结合物的生物特性作初步分析,结果表明丝胶-胰岛素结合物的回收率为45.3%,体外试验表明丝胶修饰的胰岛素结合物降解速度低于牛血清(BSA)修饰的胰岛素结合物,体外半衰期是天然胰岛素的2.8倍。

s: With natural silk protein as raw material, the sericin protein powder which is water-solubleand whose molecular weight is 1～70KD can be produced by hydrolysis under high temperature and high-pressure or by acid degradation.With glutaraldehyde as cross-linking agent, the sericin protein isconjugated with insulin to gain sericin-insulin conjugate (SS-Ins in short).With Enzyme-Linked ImmunosorbentAssay (ELISA),the biological properties of the conjugate are analyzed primarily, and the results showthat the recovery efficiency of SS-Ins is 45.3%.The in vitro experiment indicates that the degradationvelocity of the insulin modified with sericin is much lower than that with BSA and the external half-lifeis 2.8 times as much as that of natural insulin.