太原市HBsAg阳性孕妇新生儿HBV感染状况的研究

Infection status of HBsAg positive pregnant women's newborns in Taiyuan city

目的 了解目前太原市HBsAg阳性孕妇新生儿HBV感染状况。方法 ELISA法检测新生儿外周血HBsAg ;巢式聚合酶链反应 (nPCR)检测新生儿外周血HBVDNA ;选择性聚合酶链反应 (sPCR)检测外周血单个核细胞 (PBMC)HBVDNA ,其中任一项阳性即判为发生新生儿HBV宫内感染。结果 15 1例HBsAg阳性孕妇所生新生儿 ,ELISA法检测 5例HBsAg阳性 ,nPCR检测 2 9例HBVDNA阳性 ,sPCR检测 36例PBMCHBVDNA阳性 ,新生儿任一项阳性者 5 7例 ,合计宫内感染率37.75 % (5 7/15 1)。结论 检测PBMC内HBVDNA是对外周血HBVDNA的一个重要补充 ,太原市HBsAg阳性孕妇新生儿HBV宫内感染率 37.75 % ,与国内外同类研究结果相比 。

Objective To find out the infection status of HBsAg positive pregnant women' newborns in Taiyuan city. Methods HBsAg was determined by ELISA. HBV DNA was determined by nested PCR and PBMC HBV DNA was determined by selected PCR. The newborns were regarded as intrauterine infectious if any of the 3 markers was positive. Results The total HBV intrauterine infection rate was 37.75% . HBV intrauterine infection rates were 3.31% for HBsAg , 19.21% for HBV DNA and 23.84% for PBMC HBV DNA in newborns,respectively. Conclusion The detection of PBMC HBV is an important sumplement to the detection of serum HBV DNA. HBV intrauterine infection rate of HBsAg positive pregnant women's newborns is 37.75% in Taiyuan city, and it is lower compared with other study results.