大黄对ANP时肝细胞损伤的防治作用的实验研究

Preventative and therapeutic effects of rhubarb on hepatocyte injury in acute necrotizing pancreatitis

目的 探讨大黄对急性坏死性胰腺炎 (ANP)时肝细胞损伤的防治作用。方法 将 6 0只SD雄性大鼠随机分为大黄治疗组、盐水治疗组和对照组 ,每组 2 0只。将大黄治疗组、盐水治疗组大鼠制成ANP模型 ,对照组仅行胰胆管逆行穿刺。大黄治疗组于制模后用 2 0 0mg/kg大黄混悬液 1ml灌胃 ,盐水治疗组和对照组应用等体积的生理盐水灌胃 ,每 6h 1次。三组分别于术后 0、1、3、6和 12h取肝组织 ,HE染色观察肝组织的病理变化 ,TUNEL法测定肝细胞凋亡指数 ,电镜观察肝细胞超微结构的改变。结果 大黄治疗组肝细胞病理变化显著轻于盐水治疗组 ,凋亡指数明显少于盐水治疗组 (P <0 .0 5 ) ,肝细胞的超微结构改变较盐水治疗组轻。

Objective To investigate the preventative and therapeutic of rhubarb effect on hepatocyte injury in acute necrotizing pancreatitis. Methods Sixty male SD rats were randomly equally divided into the rhubarb treated group(Ⅰ), the saline treated group(Ⅱ) and control group (Ⅲ). Groups Ⅰ and Ⅱ and were made into ANP model, and for group Ⅲ the retrograde cholangiopancreatic duct puncture was performed punctured merely. The group Ⅰwas given the suspension of rhubarb powder in the dose of 200 mg/kg by nasotube. Groups Ⅱ and Ⅲ were given the equal volume saline by nasotube,once every 6 h. All rats' hepatic tissue in 3 groups was taken in at 0、1、3、6 and 12 h after operation,then the hepatic tissue pathological changes (HE stained) was observed, the apoptotic index of hepatocytes was assayed by the way of TUNEL and the ultrastructure change of hepatocytes was observed. Results Compared with groupⅢ, the pathological change of hepatic tissue in rhubarb treated group was slighter. The apoptotic index of hepatocytes was lesser and the ultrastructure change of hepatocytes was slighter too. Conclusion The preventative and therapeutic effect of rhubarb on injury of hepatocytes of acute necrotizing pancreatitis is remarkable.