无淋巴结转移的原发性口腔黏膜鳞癌细胞凋亡的原位观察

Apoptosis in Primary Oral Squamous Cell Carcinomas without Lymph Node Metastases.

目的 :研究原发性口腔黏膜鳞癌细胞凋亡发生情况 ,并分析其与临床分期及病理学分级之间的关系。方法 :采用末端脱氧核苷酸转移酶介导的dUTP缺口末端标记 (TUNEL)法检测凋亡细胞 ,计算凋亡指数 (apoptoticindex ,AI)。结果 :30例原发性口腔黏膜鳞癌均有凋亡细胞的阳性着色 ,AI范围为 0 .0 3～ 0 .92 ,平均AI为 0 .32。相对于肿瘤侵及范围大的鳞癌组织 ,肿瘤侵及范围小的鳞癌组织中细胞凋亡发生率较高 (P <0 .0 5 ) ;细胞凋亡发生率与病理学分级之间也有统计学意义 (P <0 .0 5 ) ,鳞癌恶性程度越高其凋亡发生率越低。结论

Objective:To investigate apoptosis in primary oral squamous cell carcinomas(OSCCs) without lymph node(LN) metastases and to study the relation between apoptosis and clinical and pathological characteristics of primary OSCCs without LN metastases.Methods:For determination of apoptosis, the terminal deoxynucleotidyl trasferase(TdT) -mediated dUTP nick end labeling(TUNEL)was used in 30 OSCC cases; the percentages of apoptotic cells among tumor cells were calculated as apoptotic index(AI).Results:In all 30 cases, apoptotic cells could be visualized by TUNEL(AI ranged from 0.03 to 0.92, average value 0.32).AI was significantly negatively correlated with T classes(P<0.05) and pathological grade(P<0.05), respectively.Conclusions:The incidence of apoptosis is related to the invasion and malignant degree of OSCCs without LN metastases.