中华蟾蜍、黑斑蛙冬眠期与非冬眠期消化管嗜银细胞的比较

Argentaffine Cells in Digestive Tract of Hibernating and Nonhibernating Bufo gargarizans and Pelophylax nigromaculata

以改良龙桂开银染法与Fortana Messon银染法制作冬眠期与非冬眠期中华蟾蜍、黑斑蛙消化管各部组织切片 ,观察、测量与比较这两种无尾两栖动物冬眠期与非冬眠期消化管嗜银细胞的形态、密度与分布。结果表明 :无论冬眠期还是非冬眠期两种动物消化管嗜银细胞的形态、分布规律相似 ,嗜银细胞的形态与其所在部位及其颗粒分泌方向有关 ,嗜银细胞在胃幽门处有明显的密度高峰 ;两种动物消化管嗜银细胞的分布范围、形状在冬眠期与非冬眠期相似 ,但冬眠期嗜银细胞的密度和嗜银细胞内的嗜银颗粒密度高于非冬眠期 。

Objective: To observe and compare the configuration and di stribution of argentaffine cells in digestive tract of hibernating and non\|hib ernating Anura Amphibians. Methods: each part of digestive tract of hibernating and non\|hibernating Bufo gargarizans and Pelophylax nigromaculata was r outinely embeded with paraffin,ferially sectioned and stained with improved LONG Guikai's and Fortana\|messon's silvermethod. The configuration, distribution an d density of the argentaffine cells in digestive tract were observed. Results an d conclusions: 1.The distribution and configuration of the argentaffine cells in digestive tract of Bufo gargarizans is similar to that of Pelophylax nigr omaculata. The configuration of argentaffine cells is related to their positio n and secreting direction of argentaffine granules. The argentaffine cells were densely populated in pylous;2.The distribution, density and configuration of the argentaffine cells in digestive tract of the two Anura Amphibians in hibernatio n period is similar to that in non-hibernation period, but the density of the a rgentaffine cells and the density of argentaffine granuals in the argentaffine c ells were higher in hibernation period than in non-hibernation period, suggesti ng that endocrinic function of the digestive tract is more reserved in hibernati on period.