耐低温木霉TR-165固态发酵条件的研究

The study on solid fermentative condition of Trichoderma TR-165

目的 为使木霉代替农药,对土壤中分离筛选的低温木霉TR 165固态发酵条件进行研究。方法 采用单因素实验。结果 在含麸皮、苹果渣及无机盐的固料中,于pH7.0,含水量20%,接种量8%(孢子液/固料),初始2d为24℃,第3～5d为20℃,第6d为26℃,固料厚度2cm,每4～5h搅拌并喷水1次,发酵7～9d,孢子密度可达1010g-1以上。结论 以麸皮、苹果渣及无机盐为固料发酵生产低温木霉是一种快速、高效和实用的方法。

Aim The fermentative condition of Trichoderma TR-165 from soil of the low tempreture area is studied. Methods Single factor experiment.ResultsIn the solid medium which include wheat bran, apple dreg and inorganic salt, fermentation processes.The optimum condition is as following: the water containing percentage is 20%; inoculum is 8%;during the fermentation ,the temperature of 1st～2nd day is 24℃, 3rd～5th day is 20℃, and after the 6th day is 26℃; the thickness of medium is 2cm ,and the medium is agitated and watered every 4～5 h; the fermentation period is 7～9 days. The conidial concentration can be over 10^(10)spores g^(-1).ConclusitonThe solid fermenting of Trichoderma TR-165 with wheat bran, apple dreg and inorganic salt is a fast, efficient and practical method