补肾壮骨丸中淫羊藿苷的高效液相色谱法测定

Determination of icariin in Bushen Zhuanggu pills by HPLC

目的 建立高效液相色谱法测定补肾壮骨丸中淫羊藿苷的含量。方法 70 %乙醇提取后 ,用聚酰胺柱纯化 ,供试品溶液在C18色谱柱上以甲醇 水 冰醋酸 (5 5∶4 5∶1)为流动相 ,2 70nm下外标法测定。结果 淫羊藿苷进样量在 0 .0 84～1.0 0 8μg内线性关系良好 (r =0 .9999) ,平均回收率为 99.4 % ,RSD 2 .2 %。 结论 本法操作简便 ,结果准确 ,可作为补肾壮骨丸质量控制的方法。

OBJECTIVE To develop an HPLC method for the assay of icariin in Bushen Zhuanggu pill. METHOD The sample was extracted with 75% ethanol followed by purification with polyamide column. The assay quantitative analysis was performed on a C 18 column with a mobile phase of methanol-water-glacial acetic acid (55∶45∶1), and detection wavelength at 270 nm. The determination was according to the external standard method.RESULTS Good linearity of icariin was showed within the range of 0.084～1.008μg (r = 0.999 9). The average recovery was 99.4%, and RSD was 2.2%.CONCLUSION The method is simple and accurate. It is suitable for the quality control of this preparation.