结内非霍奇金淋巴瘤微卫星位点BAT-26和BAT-25的分析

Microsatellite analysis of BAT-26 and BAT-25 in nodal non-Hodgkin′s lymphomas

目的 :通过微卫星位点BAT 2 6和BAT 2 5的分析 ,探讨结内非霍奇金淋巴瘤是否存在微卫星不稳定性。方法 :收集手术活检所得 51例结内非霍奇金淋巴瘤和 9例淋巴结反应性增生的冷冻标本 ,并分离基因组DNA ,通过PCR法扩增微卫星位点BAT 2 6和BAT 2 5,应用全自动DNA测序仪和GeneScan 3 .1软件进行片段分析 ,观察这两个位点重复序列长度的变化。以已知遗传性非息肉病性结直肠癌的高度微卫星不稳病例 10例为阳性对照。结果 :扩增成功的标本均未见BAT 2 6或BAT 2 5位点单碱基重复序列大小异常。结论 :采用结直肠肿瘤中高度敏感的微卫星稳定性检测位点BAT 2 6和BAT 2 5,未发现结内非霍奇金淋巴瘤存在高度微卫星不稳 。

Purpose:To investigate the existence of micro sa tellite instability in nodal non-Hodgkin's lymphomas by microsatellite analysis of BAT-26 and BAT-25.Methods:Frozen tissues of 51 nodal non-Hodgkin's lymphomas and 9 lymphoid reactive hyperplasia were collected by surgical biopsy. Genomic DNA was extracted. Microsatellite alterations of BAT-26 and BAT-25 were detected b y polymerase chain reaction(PCR)followed by fragment analysis using automatic DNA sequencer and GeneScan 3.1 software. 10 cases of hereditary nonpolyposis col orectal cancers with known high frequency microsatellite instability (MSI-H) we re retrieved as positive controls. Results:No aberration of mon onucleotide duplication in the microsatellite markers BAT-26 and BAT-25 was ob served in all successfully amplified specimens.Conclusions:The microsatellite analysis of BAT-26 and BAT-25, two highly sensitive markers to microsatellite status in colorectal tumors, demonstrated no evidence of MSI-H i n nodal non-Hodgkin's lymphomas. The existence of low frequency microsatellite instability and participation of mismatch repair genes in lymphomagenesis remain to be determined by further studies.