肿瘤坏死因子相关凋亡诱导配体联合阿糖胞苷诱导急性髓细胞性白血病细胞凋亡的研究

An experimental research of apoptosis-inducing effect of TRAIL and Ara-C on acute myeloid leukemic cells

目的 :探讨肿瘤坏死因子相关凋亡诱导配体 (TRAIL)联合阿糖胞苷 (Ara C)诱导急性髓细胞性白血病 (AML)细胞凋亡的作用。方法 :分离 39例AML患者的骨髓单个核细胞 ,每例分为 4组进行观察 :空白对照组、TRAIL组、Ara C组、TRAIL加Ara C组 ,共同培养 12、2 4和 4 8h。通过MTT比色法测定细胞杀伤率及流式细胞仪检测细胞凋亡。结果 :TRAIL和Ara C可协同降低AML细胞活力 ,抑制细胞增殖 ,诱导细胞凋亡 ,其作用随培养时间延长及药物浓度增加而增强。结论 :TRAIL与Ara C具有协同作用 ,能高效杀灭肿瘤细胞 。

Objective:To investigate the apoptosis-inducing effect of TRAIL and Ara-C on acute myeloid leukemic(AML) cells. Methods:Thirty-nine cases of BMMNC of AML cells were isolated. All cases were divided into 4 groups, which were control group, TRAIL group, Ara-C group and TRAIL+ Ara-C group.MTT assays were used to evaluate the cytotoxic effect at 12, 24 and 48 hours after the drugs were added. Flow cytometry was used to evaluate apoptosis. Results:TRAIL and Ara-C can synergistically inactivate AML cells, and induce apoptosis. This kind of effect presented a dose-and time-dependent relationship.Conclusion:TRAIL can present a synergistic effect with Ara-C.The cytotoxic effect was mostly induced by apoptosis. TRAIL has a promising prospect in clinical use.