中华眼镜蛇毒活性组分抑血管内皮细胞增殖和黏附实验研究

The Inhibiting Effect in Vascular Endothelial Cells' Growth and Adhesion of Active Fraction Isolated from Naja Naja Atra Venom.

目的:研究眼镜蛇毒在抗血管生成方面的生物活性。方法:采用Sephadex阳离子交换柱和分子筛分离中华眼镜蛇毒原毒,以MTT法评价和筛选分离各蛋白峰对培养内皮细胞的抑生长活性;MTT快速测定法测定活性峰对内皮细胞与纤维蛋白原(Fbg)、纤连蛋白(Fn)、Matrigel的黏附作用的影响。结果:经两步分离后从中华眼镜蛇毒中获得一可特异性抑制内皮细胞生长的活性蛋白峰,该活性组分可抑制内皮细胞的黏附功能,对细胞黏附Fn、Fbg和Matrigel等3种物质的作用从1.2μg/ml浓度起即有抑制效应(P<0.05),且均呈浓度依赖性;组分对细胞黏附Fbg和Hatrigel的抑制作用较其对Fn的作用强(P<0.05)。结论;眼镜蛇毒中可能含有可抑制血管生成的活性物质,在抗血管生成方面有良好的研究价值。

Objective: To investigate the anti-angiogensis activity of naja naja venom. Methods: Isolated naja naja crude venom by CM-Sephadex column and Sephadex-G column, then selected the vascular endothelial cell' s (VEC's) growth-inhibiting active fraction by MTT assay. The inhibition effect of the selected fraction in cell's adhesion to fibronectin. fibrinogen and matrigel was also detected. Results: An active fraction was obtained from naja naja crude venom, which can specially inhibit VEC's growth and adhesion to fibronectin, fibrinogen and matrigel in a dose-dependent manner(P < 0. 05). Conclusion: Maybe there is an active anti-angiogenesis fraction in naja naja venom which will be worth studying further as a new drug in some angiogenie diseases therapy m future.