消旋聚乳酸/羟基磷灰石/脱钙骨基质材料支架的体外细胞相容性实验研究

Biocompatibility of PDLLA/HA/DBM with human osteoblasts co-cultured in vitro

目的 研究一种新的骨替代材料———消旋聚乳酸 羟基磷灰石 脱钙骨基质 (PDLLA HA DBM)骨细胞界面形态 ,评价其生物相容性。方法 采用体外细胞培养技术 ,选用培养的人成骨细胞 ,通过光镜和扫描电镜等观察 ,了解细胞在材料中的生长情况和相互关系 ,并与消旋聚乳酸 (PDLLA)及脱钙骨 (DBM)相比较。结果 随着培养时间的延长 ,细胞在PDLLA HA DBM材料表面的密度增加 ,未见细胞死亡。细胞以锚状结构牢固地附着于材料表面 ,并分泌细胞外基质 ,覆盖并完全包裹材料颗粒。结论 PDLLA HA DBM与人成骨细胞能早期形成骨性结合界面 ,具有良好的生物相容性 ,可望成为一种新的具有前景的骨替代材料。

Objective To evaluate the osteocompatibility of poly(D,L lactic acid)/ hydroxyapatite/decalcified bone matrix (PDLLA/HA/DBM) and to compare the osteocompatibility with that of PDLLA and DBM. Methods The isolated human osteoblasts from the femoral head of patients were inoculated onto PDLLA/HA/DBM, PLA, and DBM. The interface between biomaterial and osteoblasts was investigated with phase contrast microscope and scanning electron microscope. Results Osteoblasts were attached tightly to the surface of biomaterial with an arched structure and had normal morphology. The extracellular collagenous matrix covered the surface of biomaterial and packed the granules of biomaterial completely. Conclusion PDLLA/HA/DBM can form osteointegrated interface at the early stage with good biocompability.