烧伤后假上皮瘤肉芽肿样病变内毒素测量及其致病机制

AIM: To investigate the content of endotoxin in Pseudo epitheliomatous hyperplastic granuloma like lesions and its pathogenic mechanism. METHODS: Endotoxin concentrations in the tissue were measured with chromogenic limulus amebocyte lysate (LAL), which was modified by perchloric acid (PCA) pretreatment for samples. To observe the effect of supernate from peripheral blood mononuclear cells by single or repeated endotoxin stimulation. on proliferation of cultured fibroblasts peripheral blood mononuclear cells. RESULTS: Endotoxin was detected in the injured skin but not detected in the normal skin. Supernate from rom peripheral blood mononuclear cells by single endotoxin stimulation has stronger effect on proliferation of fibroblats than repeated stimulation. CONCLUSION: Pseudo epitheliomatous hyperplastic granuloma like lesions were resulted from the bacteria in the injured shin, the bacteria produced endotoxin which stimulated the local inflammatory cells to produce cytokines,and the cytokines led to the overgrowth of repairing cells in the skin.