转录因子GATA-3和白细胞介素5在鼻息肉中的表达及其相关性

Relationship between expressions of GATA-3 and interleukin-5 in nasal polyps

目的 探讨转录因子GATA 3在鼻息肉发病中的作用。方法 采用免疫组织化学和逆转录多聚酶链反应 (RT PCR)试验检测了 2 8例慢性鼻窦炎患者鼻息肉和 17例正常人鼻粘膜中GATA 3的表达 ,同时采用酶联免疫吸附试验检测了白细胞介素 5 (IL 5 )在标本中的表达情况。结果 2 8例鼻息肉中 2 5例GATA 3免疫组织化学染色均呈阳性 ,阳性率 89 3% ,阳性部位主要位于粘膜下炎症细胞内 ;正常鼻粘膜中 5例染色阳性 ,阳性率 2 9 4 %。所有病理组织RT PCR均获得预期结果 ,PCR反应后患者鼻息肉中GATA 3相对密度为 0 6 2± 0 14 ,正常鼻粘膜中GATA 3相对密度为0 2 1± 0 11。鼻息肉中IL 5的含量为 6 9 4± 15 1pg/mg ,正常鼻粘膜中IL 5的含量为 2 5 7± 13 0pg/mg。鼻息肉中GATA 3和IL 5的表达呈显著的正相关。 结论 GATA 3参与了鼻息肉形成过程中细胞因子IL 5等的过度表达 ,对鼻息肉的形成具有重要的意义。

Objective To evaluate the role of transcription factor GATA-3 in the pathogenesis of nasal polyps. Methods The expression of GATA-3 was detected by immunohistochemistry and reverse transcriptase polymerase chain reaction(RT-PCR) in 28 cases of nasal polyps and 17 specimens of normal nasal epithelium. The expression of interleukin-5 (IL-5) in these specimens was detected by enzyme-linked immunosorbent assay (ELISA) meanwhile. Results Immunohistochemistry showed, as confirmed by RT-PCR, a positive rate of GATA-3 of 89.3% in the nasal polyp(25/28) and 29.4% in the normal nasal mucosa(5/17), P <0.05. GATA-3 was mainly distributed in submucous inflammatory cells. The relative density ratio of GATA-3 to GAPDH was 0.618±0.137 in nasal polyp and 0.21±0.11 in normal nasal mucosa ( P <0.05) as indicated by RT-PCR and agarose electrophoresis. The concentrations of IL-5 were 69.4±15.1 pg/ml and 25.7±13.0 pg/mg in the two groups respectively. The expression of GATA-3 was positively correlated to the expression of IL-5 in the nasal polyp. Conclusion Contributing to the overexpression of such cytokines as IL-5, GATA-3 may play an important role in the pathogenesis of nasal polyps.