蒙花忍冬的组织培养与快速繁殖研究

Tissue culture and rapid propagation of Lonicera japonica

以金银花 蒙花 品系的茎段为外植体 ,于 MS和 B5附加不同激素配比的培养基上 ,探讨愈伤组织形成和丛生芽诱导及生根培养的条件 .诱导愈伤组织和丛生芽的培养基为 B5+BA2 .0 m g· L- 1 +KT0 .2～ 0 .5 mg· L- 1+IAA 0 .5～ 1 .0 mg· L- 1 +L H 1 0 0 0 m g· L- 1 ,继代增殖培养的培养基为 B5+BA 2 .0 m g· L- 1 +KT 0 .5 m g·L- 1 +IAA1 .0 m g· L- 1 +L H1 0 0 0 m g· L- 1和 B5+BA2 .0 mg· L- 1 +KT0 .36 m g· L- 1 +IAA1 .0 mg· L- 1+CH 1 0 0 0 m g· L- 1 +1 / 2 MS :大量元素 ,生根培养基为 1 / 2 MS +IBA 0 .2 mg· L- 1 +L H 1 0 0 0 mg·L- 1 .结果表明 L H和

The stem segments from japanese honegsuckle cultivar Menghua( Lonicera japonica ), as the explants were cultured on MS and B 5 media composed of different phytohormones. The differentiation and growth of callus, buds and shoots were discussed. The results showed the callus , buds and shoots were easy to be induced and grew rapidly on B 5 + BA 2.0 mg·L -1 + KT 0.2～0.5 mg·L -1 + IAA 0.5～1.0 mg·L -1 + LH 1 000 mg·L -1 ; however the propagated coefficient was high on B 5 + BA 2.0 mg·L -1 + KT 0.5 mg·L -1 + IAA 1.0 mg·L -1 + LH 1 000 mg·L -1 and B 5 + BA 2.0 mg·L -1 + KT 0.36 mg·L -1 + IAA 1.0 mg·L -1 + CH 1 000 mg·L -1 + 1/2 MS(Ⅰ); and roots were easy to form on 1/2 MS + IBA 0.2 mg·L -1 + LH 1 000 mg·L -1 .