消瘢醑对瘢痕疙瘩成纤维细胞I、III型胶原蛋白的影响

Effect of Xiao Ban Xu on the expression of collagenⅠand Ⅲ of keloid fibroblasts in vitro

目的 研究复方中药制剂消瘢醑对体外培养的瘢痕疙瘩成纤维细胞 (Keloidfibroblast,KFB)I、III型胶原蛋白的影响。 方法 6例瘢痕疙瘩成纤维细胞为实验组 ,6例正常皮肤成纤维细胞 (Normalfibroblast,NFB)为对照组 ,采用成纤维细胞体外培养、ABC免疫细胞化学染色技术及积分光密度 (IOD)分析 ,观察在 10 μg ml消瘢醑浓度作用下 ,瘢痕疙瘩和正常皮肤成纤维细胞I、III型胶原蛋白的表达。 结果 瘢痕疙瘩成纤维细胞I、III型胶原阳性染色表达强度均显著高于正常皮肤成纤维细胞 (11113 1± 130 4 9vs 35 19 6± 2 36 0与 1115 7 7± 130 0 3vs 2 6 2 6 5± 4 2 6 3,t值分别为 14 4 2和 13 4 7,P值分别为 0 0 0 0 0 3和 0 0 0 0 0 4 )。 10 μg ml浓度的消瘢醑作用 4 8小时后 :1.瘢痕疙瘩成纤维细胞I、III型胶原阳性染色表达强度均显著高于正常皮肤成纤维细胞 (76 75 4± 82 5 5vs 2 30 5 2± 32 0 4与 10 5 95 2± 1311 5vs 2 4 34 8± 35 6 9,t值分别为 13 37和 12 6 6 ,P值分别为0 0 0 0 0 4和 0 0 0 0 0 5 ) ;2 .瘢痕疙瘩和正常皮肤成纤维细胞呈阳性表达的I、III型胶原染色强度明显下降 ,与未经药物处理的相应空白对照相比具有显著性差异 (76 75 4± 82 5 5vs 11113 1± 130 4

Objective To explore the effect of Xiao Ban Xu, a compound Chinese traditional medicinal prescription, on the expression of collagenⅠand Ⅲ of keloid fibroblasts in vitro. Methods Six samples of keloid fibroblasts (KFB) and 6 samples of normal fibroblasts (NFB) entered the study as experimental and control groups, respectively. With the application of Xiao Ban Xu (10μg/ml), the fibroblast culture system and the ABC immunocytochemical staining method were used to investigate the expression of collagenⅠand Ⅲ of KFB and NFB. Results The density of the staining of collagenⅠand Ⅲ in the experimental group was much higher than that in the control group, with (7675 4±825 5 vs 2305 2±320 4 and 10595 2±1311 5 vs 2434 8±356 9; t =13 37 and 12 66, P =0 00004 and 0 00005) or without (11113 1±1304 9 vs 3519 6±236 0 and 11157 7±1300 3 vs 2626 5±426 3; t =14 42 and 13 47, P =0 00003 and 0 00004) the addition of Xiao Ban Xu. Compared with blank control group, the density of the staining of collagenⅠand Ⅲ was degenerated greatly in the experimental group after 48 hours of application of Xiao Ban Xu (7675 4±825 5 vs 11113 1±1304 9 and 10595 2±1311 5 vs 11157 7±1300 3; t =10 31 and 4 68, P =0 0001 and 0 0054). Furthermore, the inhibition ratios of collagenⅠwas 30 7%, and the one of collagen Ⅲ 5 1%, in the experimental group. Conclusions The expression of collagenⅠand Ⅲ of keloid fibroblasts in vitro may be significantly more intensive than the one of normal skin fibroblasts. It seems that the expression of collagenⅠand Ⅲ, especially collagenⅠ, in keloid fibroblasts and normal skin fibroblasts in vitro may be suppressed greatly by the application of Xiao Ban Xu.