摘要
The spontaneous changes in cell shape and size, the membrane bending elastic modulus, and the structureand functions of intracellular hemoglobin of single humanliving erythrocyte incubated in different suspension pH were studied. The non-invasive, in situ, real time measurements on the parameters at the three levels of intracellular molecule, membrane and cell morphology were performedsimultaneously by using a fast micro-image analyzingtechnology and a multi-channel micro-spectrophotometer system. The result shows that the cell shape and size, themechanical property of the cell membrane, and the structure and function of intracellular hemoglobin are correlated and affected by each other with pH.
The spontaneous changes in cell shape and size, the membrane bending elastic modulus, and the structureand functions of intracellular hemoglobin of single humanliving erythrocyte incubated in different suspension pH were studied. The non-invasive, in situ, real time measurements on the parameters at the three levels of intracellular molecule, membrane and cell morphology were performedsimultaneously by using a fast micro-image analyzingtechnology and a multi-channel micro-spectrophotometer system. The result shows that the cell shape and size, themechanical property of the cell membrane, and the structure and function of intracellular hemoglobin are correlated and affected by each other with pH.
基金
supported partly by the National Natural Science Foundation of China(Grant No.30227001).
关键词
PH值
红血细胞
血红蛋白
形态学
弹性模数
erythrocyte membrane, hemoglobin, bending elasticmodulus, absorption spectrum, pH.
