缺血再灌注损伤对大鼠视网膜功能的影响

Effect of ischemia-reperfusion injury on the retinal functions of rats

目的 探讨缺血再灌注损伤对大鼠视网膜功能的影响。 方法 70只健康 Wistar大鼠 ,预实验随机抽取 2 0只分为正常对照组和单纯灌注组 ,记录视网膜电图 (electroretinography,ERG)并测定 b波峰值 ,经统计学处理无差异后 ,其余 5 0只随机分为 10组 ,用升高眼压 1h的方法建立右眼视网膜缺血模型 ,分别于缺血后 1h及再灌注 3、6、12、2 4 h、3、5、7、14、2 1d记录双眼暗视闪光 ERG并测定 b波峰值。 结果 正常对照组动物左右眼 ERG b波峰值无差异 ;单纯灌注眼与正常对照眼 ERG b波峰值无差异 ;单纯缺血组实验眼 ERG各波消失 ,再灌注实验眼组 ERG b波部分恢复 ,但随再灌注时间的延长 b波峰值呈进行性下降。 结论 视网膜缺血再灌注损伤可导致大鼠视网膜功能持续渐进性的影响。

Objective To explore the effect of ischemia-reperfusion injury on the retinal functions of rats. Methods Seventy Wistar rats were selected, 20 of which were selected randomly and divided into two groups (control group and single-irrigated group). The rats were anesthetized and their anterior chambers of the right eyes were cannulated with a 7-gauge needle connected to a reservoir containing ringers balanced salt solution, which was maintained at the same level of the eye for 1 hour. After that, ERG was recorded in both eyes of all rats. All the left rats were divided randomly into 10 groups and they were treated as the single-irrigated group. Retinal ischemia was induced by raising the reservoir to a height of 150 mm Hg. One hour later except the single ischemia group, all of the groups resumed perfusion after 3,6,12,and 24 hours and 3,5,7,14,and 21 days separately. ERG was recorded in both eyes of all rats. Results There was no difference in the results of ERG between left and right eyes in either the control group or the single-irrigated group. All the waves of ERG vanished in the single-ischemia group after 1 hour. In the ischemia-reperfusion groups, the waves of ERG partly recovered and the amplitude reduced persistently and progressively. Conclusion Ischemia-reperfusion injury may affect the function of the retina persistently and progressively.