摘要
目的 探讨大鼠视神经损伤后 Nogo- A m RNA的表达变化。 方法 采用逆转录 -聚合酶链反应 (reverse transcription polymerase chain reaction,RT- PCR)法 ,半定量分析视神经损伤后 3、7、9、15、2 1、2 5 d视神经组织 Nogo- A m RNA表达量的变化。 结果 大鼠正常视神经组织中表达 Nogo- Am RNA,但表达量较低 ;损伤后 3d视神经 Nogo- A m RNA表达量显著升高 ,至伤后 2 5 d仍维持较高的水平。 结论 视神经损伤后 Nogo- A m RNA表达升高。
Objective To evaluate the expressive varieties of Nogo-A mRNA in injured optic nerves of rats. Methods Reverse transcription polymerase chain reaction (RT-PCR) method was used to hemi-quantitatively analyze the levels of Nogo-A mRNA in the optic nerves 3, 7, 9, 15, 21, and 25 days respectively after injury. Results The level of the expression of Nogo-A mRNA was low in the normal optic nerves, while it was significantly high in the optic nerves 3 days after injury, and kept the high level still after 25 days. Conclusion The expression of Nogo-A mRNA in injured optic nerves is increased.
出处
《中华眼底病杂志》
CAS
CSCD
2003年第4期247-249,共3页
Chinese Journal of Ocular Fundus Diseases
基金
国家自然科学基金资助项目 (30 2 70 4 58)
军队"十五"医学科研基金资助项目 (0 1 MA1 75)
关键词
视神经损伤
NOGO-A
MRNA表达
神经生长因子
逆转录聚合酶链反应
Optic nerve Injury
Nerve growth factor/antagonists & inhibitors
Reverse transcritase polymerase chain reaction
Disease models, animol