结内非霍奇金淋巴瘤编码区单碱基重复序列分析

Microsatellite analysis of coding mononucleotide repeats in nodal non-Hodgkins lymphomas

目的 在结内非霍奇金淋巴瘤 (NHL)中进行编码区单碱基重复序列长度变化分析及其与DNA错配修复相关性的研究。方法 收集活检所得 5 1例结内非霍奇金淋巴瘤和 9例淋巴结反应性增生的冷冻标本 ,并分离基因组DNA ,通过荧光PCR法扩增 ,应用全自动DNA测序仪和GeneScan 3 1软件进行片段分析 ,观察编码区单碱基微卫星位点TGF βRⅡ、IGFⅡR、BAX、hMSH3和hMSH6大小的变化。结果 仅 1例T 淋巴母细胞性淋巴瘤出现TGF βRⅡ长度变化 ,无其它异常。 结论 结合前期BAT 2 6和BAT 2 5的分析结果 ,微卫星不稳定性和编码区单碱基重复序列变化在结内NHL中都较少见。

Purpose To investigate the length alterations of coding mononucleotide repeats in nodal non Hodgkins lymphomas and the relationship between the disease and DNA mismatch repair genes. Methods Frozen tissues of 51 nodal non Hodgkins lymphomas and 9 lymphoid reactive hyperplasia were collected by surgical biopsies. Genomic DNA was extracted. Size changes in coding mononucleotide repeats of TGF βRⅡ, IGFⅡR, BAX, hMSH3 and hMSH6 were retrospectively detected by fluorescent polymerase chain reaction (PCR) followed by fragment analysis using automatic DNA sequencer and GeneScan 3 1 software. Results Microsatellite alteration of TGF βRⅡ was found in only one T lymphoblastic lymphoma, with no positivity revealed in all other informative cases. Conclusion The combined results with the previous microsatellite analysis of BAT 26 and BAT 25, indicate that both MSI and coding mononucleotide repeats alterations are rare in nodal non Hodgkin's lymphomas.