rSj14-3-3和rSj14-3-3/SjGST对日本血吸虫虫卵肉芽肿形成的影响

Effects on liver granuloma in mice immunized with rSj14-3-3 and rSj14-3-3/SjGST after experimental infection with Schistosoma japonicum

目的 探讨日本血吸虫信号蛋白 14 - 3- 3基因重组蛋白 (rSj14 - 3- 3)及其与谷胱甘肽S转移酶融合蛋白(rSj14 - 3- 3/SjGST)对宿主肝虫卵肉芽肿形成的影响。 方法 用rSj14 - 3- 3和rSj14 - 3- 3/SjGST免疫雌性BALB/c小鼠 ,末次免疫后 5d ,各组小鼠均感染日本血吸虫尾蚴 4 0± 1条 /鼠 ,6周后 ,剖杀 ,取肝组织计数肝表面虫卵结节密度和肝切片上单个虫卵肉芽肿直径大小。结果 免疫组 (14 - 3- 3组和 14 - 3- 3/GST组 )和对照组肝表面虫卵结节数分别为 6 72±1 14、5 89± 1 0 3和 2 1 0 5± 1 2 6 ,前两者比后者分别减少了 6 8 0 8%和 72 0 2 % ;肝肉芽肿平均直径免疫组为 178 12±32 18μm和 14 8 13± 2 9 6 5 μm ,与对照组 2 75 0 0± 38 2 1μm相比分别减少了 35 2 3%和 4 6 13%。结论 重组抗原rSj14 - 3- 3和rSj14 - 3- 3/SjGST对日本血吸虫具有较好的抗病效果。

Aim To observe the anti-granuloma effects of recombinant Sj14-3-3 and Sj14-3-3/SjGST against Schistosoma japonicum.Methods Female BALB/c mice were immunized with rSj14-3-3 and rSj14-3-3/SjGST.Five days after final injection,mice of each group were challenged with 40±1 S.japonicum cercariae.All the mice were killed in the 6th week after challenge so as to calculate the density of egg node in the surface of liver and estimate the egg granuloma size on the slide of liver.Results The numbers of egg node of rSj14-3-3 group,rSj14-3-3/SjGST group and control group were 6.72±1.14,5.89±1.03 and 21.05±1.26 respectively.The former two were decreased by 68.08% and 72.02%;the mean granuloma sizes were decreased by 35.23% and 46.13% with 178.12±32.18μm and 148.13±29.65μm in two immunized groups and 275.00±38.21μm in control group.Conclusion The two recombinant antigens rSj14-3-3 and rSj14-3-3/SjGST have a rather good effect on anti-granuloma formation of Schistosomiasis japonica.