PCR-SSCP检测急性白血病患者N-ras基因突变及临床意义初探

Clinical significance of the detection of N-ras gene mutation in patients with acute leukemia by PCR-SSCP

目的 探讨N ras基因突变与急性白血病发生的关系。方法 采取骨髓和外周血白细胞提取DNA ,用PCR SSCP分析技术分别对急性白血病患者、正常对照组的N ras基因突变进行检测。结果 84例急性白血病患者中 2 5例发生N ras基因突变 ,基因变异频率为 2 9 8% ,其中急性淋巴细胞白血病为 18 6% ,急性髓细胞白血病为 41 4% ;11例随访 6个月 ,7例N ras基因突变消失 ,4例N ras基因突变持续存在 ;正常对照组未发现N ras基因突变。结论 N

Objective To explore the relationship between N ras mutation and the pathogenesis of acute leukemia. Methods Human genomic DNA was extracted from normal human peripheral white blood cell and bone marrow of patients. N ras gene mutations in 84 cases of acute leukemia and 50 normal healthy cases were detected by PCR SSCP technique, respectively. Results N ras gene mutations were found in 25 cases out of 84 cases of acute leukemia with the frequency of N ras gene variation being 29.8%, including 18.6% of acute lymphoblastic leukemia (ALL) and 41.4% of acute myelocytic leukemia(AML). After a follow up of 11 cases for 6 months, disappearance of N ras gene mutation was found in 7 cases but continuous presence of N ras gene mutation was found in 4 cases. However, no N ras gene mutation was found in 50 normal healthy cases. Conclusion N ras gene mutation is of important significance in the prognosis of patients with acute leukemia.