大鼠氮能神经与胃电节律失常的关系

Nitrergic nerves and electrogastric dysrhythmia in rats

目的 :探讨一氧化氮与胃电节律失常的关系 .方法 :2 8只大鼠随机分为 3组 :正常对照组 (12只 )和胃电节律失常模型组 (8只 )喂养 4wk后 ,先记录胃电、再行胃窦肌间神经丛氮能神经染色 ;药物组 (8只 ) :观察注射不同剂量硝普钠 (SNP)、一氧化氮合酶抑制剂左型精氨酸甲酯 (L NAME)后的胃电变化 .结果 :药物组大鼠注射硝普钠或大剂量L NAME后出现了明显的胃电节律失常 ;模型组大鼠胃电节律失常增加 ,异常节律指数 (ARI)和慢波频率变异系数 (coefficientofvariation,CV)分别为 2 1.8%和 2 5 .7% ,明显高于正常对照组大鼠 (ARI和CV分别为 9.5 %和 17.2 % ,P <0 .0 1) ,胃窦肌间神经丛的氮能神经含量也明显多于正常对照组 (2 1.9%vs13.8% ,P <0 .0 1) .结论

AIM: To investigate the relation of nitrerigic nerves and electrogastric dysrhythmia. METHODS: 28 Wistar rats were randomly divided into three groups: control group; model group of electrogastric dysrhythmias and drug group. In the rats of control group and model group, electrogastrogram was recorded and nitriergic nerves in the myenteric plexus of rat antrum were observed. In the drug group, electrogastrogram was recorded after the rats were injected intrapertoneally different dosage SNP or N(omega) nitro arginine methyledter (L NAME). RESULTS: In the rats of model group, the abnormal rhythm index (ARI) was 21.8% and the coefficient of variation of slow wave frequency (CV) was 25.7%, which were higher than that of the control group (ARI was 9.5% and CV was 17.2%, P <0.01). The content of nitrergic nerves in the myenteric plexus of model rat antrum increased compared with that of control group (21.8% vs 13.8%, P <0.01). When normal rats were injected SNP or higher dosage L NAME, electrogastric dysrhythmia increased and the abnormal rhythm index (ARI) and the coefficient of variation of slow wave frequency (CV) were significantly increased. CONCLUSION: The increase or decrease of nitric oxide can lead to electrogastric dysrhythmias.