hBMP-7基因直接体内转染修复兔桡骨缺损实验研究

A Study of the Repairing of Rabbit Radius Segmental Bone Defect Using hBMP-7 Gene Transfer in Vivo

目的 探讨含人骨形态发生蛋白 7(hBMP - 7)基因逆转录病毒液与左旋聚丙交酯 (PLLA)生物材料复合后修复兔桡骨缺损的效果。方法 制备含hBMP - 7真核细胞表达载体的逆转录病毒液PT -PLNCX2 -BMP7。制备新西兰大白兔桡骨 1 5cm缺损模型 ,修复实验分为 4组 :PT -PLNCX2 -BMP7+PLLA修复组 ,空载体 (PT -PLNCX2 ) +PLLA修复组 ,单纯PLLA生物材料修复组 ,空白组。每组 6个样本。分别于术后 8、12、16周进行大体观察、X线检测和组织学检测。结果 PT -PLNCX2 -BMP7重组病毒液与生物材料复合修复组可见在骨缺损处有骨性连接形成 ,组织学观察见有大量新生骨组织形成 ,而PT -PLNCX2 病毒液修复组、单纯材料修复组和未修复组均未见骨性连接形成 ,仅在部分骨缺损两断段形成少量骨组织。结论 利用hBMP - 7基因体内局部。

Objective To detect the ability of PLNCX 2-hBMP-7 virus granules composited with PLLA(poly L lactide)biomaterials to enhance segmental bone defect healing of the rabbit radius.Methods Human BMP-7 gene vector was reconstructed in retroviral vector.Segmental 1 5 cm defects were created surgically in the radius of New Zealand white rabbits. The Poly L lactide (PLLA)was used alone,in conjunction with PLNCX 2-hBMP-7 retroviral granules, PLNCX 2 retroviral granules to repair the defects.The defect repairing capability for each of the treatment modalities were assessed by gross observation ,radiographically, histological.Results All defects repaired by PLNCX 2-hBMP-7 retroviral granules had healed radiographically at 16 weeks postoperatively compared with no defects healed in the other groups. Histological analysis of the specimens revealed that defects that had received PLNCX 2-hBMP-7 retroviral granules were filled with coarse trabecular bone whereas in those that had received PLNCX 2, PLLA materials alone were fibrous tissue.Conclusion Dirct,local retroviral delivery of hBMP-7 may led to the healing of segmental bone defect in rabbits.