p14^(ARF)与p53抑癌基因对胰腺癌生物学行为的影响

The relationship between tumor suppressor genes p14 ARF and p53 expression and biological behavior of pancreatic carcinoma

目的 探讨胰腺癌组织中p14 ARF、p5 3的表达与胰腺癌生物学行为的关系 ,为胰腺癌的基因干预治疗提供理论依据。方法 免疫组织化学Envision改良法、ABC免疫组化法检测 10例正常胰腺组织、4 2例胰腺癌组织中p14 ARF、p5 3蛋白的表达。结果 正常胰腺组织中p14 ARF的表达率为90 % ,胰腺癌组织中为 35 7% ;正常胰腺组织中 p5 3的表达率为 0 ,胰腺癌组织中为 4 5 2 %。胰腺癌组织中 p14 ARF、p5 3的表达与胰腺癌肿块的大小、淋巴结转移率、病理分级、临床分期等相关 ,而与肿块的局部浸润无关。结论 抑癌基因 p14 ARF、p5 3与胰腺癌的发生、发展有着密切的关系 ,可以作为基因干预治疗胰腺癌的一个理论依据。

Objective To investigate the relationship between tumor suppressor genes p14 ARF and p53 expression and the biological behavior of pancreatic carcinoma. Methods Modified Envision method of immunohistochemistry was used on 42 specimens of pancreatic adenocarcinoma and 10 normal specimens of normal pancreas resected during operation to examine the expression of the gene p14 ARF . and ABC immunohistochemical technique was used to examine the expression of p53. Results The positive rates of p14 ARF in of normal pancreatic tissues and pancreatic carcinoma were 90% and 35.7% respectively ( P <0.01). The positive rates of p53 in tissues of normal pancreatic and pancreatic carcinoma were 0 and 42.5% ( P <0.05) respectively. Significant correlation was detected between the expression of p14 AR and p53 and the diameter of tumor, the rate of lymph node metastasis, pathological grade, and clinic stage in pancreatic carcinoma. No correlation was found between the expression of p14 ARF and p53 and the tumor infiltration in pancreatic carcinoma. Conclusion The tumor suppressor gene p14 ARF and p53 are closely related to the occurrence and development of pancreatic carcinoma. It is possible to treat pancreatic carcinoma by gene intervention.