具有免疫活性多糖海洋细菌菌株的筛选

Screening of marine bacterial strain with immunoactive exopolysaccharide

从厦门海区潮间带的动植物体及底泥分离到的177株海洋细菌中筛选出一株高产胞外多糖的1202菌株.其产生的胞外多糖可显著地促进昆明种小鼠脾淋巴细胞IL 2(白介素 2)的合成,并对小鼠S180肉瘤具有较强的抑制作用.经初步分类鉴定,确定该菌株归属于土壤杆菌属(Agrobacteriumsp.).

An exopolysaccharide-producing strain 1202 was obtained by screening 117 marinebacteria isolates, which were from the flora ,the fauna and the sediments in the intertidal zone of the Xiamen coast. The exopolysaccharides produced by the bacterial strain 1202 could stimulate interleukin 2 (IL-2) secretion from mouse spleen lymphocytes in vitro, and exhibit significant inhibitory effects on sarcoma 180 solid tumors in vivo. The strain 1202 was tentatively identifiedas a species of the genus Agrobacterium, based on its morphology, growth features, physiological and biochemical characteristics.