摘要
用酚 水法从20dm3溶藻弧菌培养液中分离得到脂多糖(LPS)97.6mg,以0.5mg cm3的LPS溶液通过耳缘静脉注射免疫实验兔,经过3次加强注射后从颈动脉放血制备抗血清.该抗血清的效价为1∶2560,对副溶血弧菌等10株细菌的交叉反应效价都较低.用免疫吸附过滤法去除血清中的非特异性成分.用吸附后的抗血清进行溶藻弧菌间接ELISA检测,其检测限为97×104个 cm3.以ELISA进行大黄鱼体内溶藻弧菌检测.结果表明:用LPS抗血清可以较为灵敏地检测大黄鱼体内的溶藻弧菌,该方法不仅可以用于诊断大黄鱼的溶藻弧菌病,也可以检测无病症带菌大黄鱼.
97.6mg lipopolysaccharide(LPS) were obtained by phenol-watermethod from 20dm^3 suspension of Vibrio alginolyticus. 0.5mg/cm^3 LPS were injected into rabbits for the sake of making anti-serum. The titer of anti-serum was 1∶2 560. The non-special component in the serum was removed by absorption. Serum after absorption was used for indirect ELISA detection. The detecting limit of V.alginolyticus was 9.7×10~4ind/cm^3. By using indirect ELISA of anti-LPS serum to detect V.alginolyticus in Pseudosciaena crocea, the results indicated that ELISA method could beused not only to diagnose the clinically diseased P.crocea, but also to recognize pathogenic bacteria carrying P.crocea.
出处
《台湾海峡》
CAS
CSCD
2004年第1期56-61,共6页
Journal of Oceanography In Taiwan Strait
基金
国家高技术研究发展计划(863计划)资助(863 819 02 12
2001AA635070)
关键词
溶藻弧菌
培养液
脂多糖
间接ELISA
大黄鱼
aquatic protection
indirect ELISA
comparativetest
Vibrio alginolyticus
Pseudosciaena crocea
