稻瘟病菌对三环唑抗药性测定方法的建立

Approach to measuring resistance of Pyricularia grisea to tricyclazole

研究了稻瘟病菌对三环唑抗药性的测定方法,确定了保绿培养基的成分,建立了抗药性测定的标准化模型。4叶I心期将稻苗在三环唑系列浓度药液中浸根处理36 h,剪取稻苗叶段插于100 μg/mL苯并咪唑+0.5％水琼脂保绿培养基上,用孢子含量为105个/mL的稻瘟病菌孢子悬浮液喷雾接种,保湿培养8 d后调查结果,计算EC50值。该测定方法与小苗测定相比较结果更加稳定且灵敏度高,重复性好。

The method of monitoring resistance of Pyricularia grisea to tricyclazole was studied and the model was set up. After root-soaking for 36 hours, the leave fragments of rice seedlings were cut and placed onto 0.5% agar with 100 μg/ mL benzimidazole. After artificially inoculated with spore suspension (105 spores/mL) , the leave fragments were put back to the moist climatic chamber for observation of disease symptoms for 8 days. Comparative studies showed that the approach described in the present paper was more stable and sensitive than the seedling treatment.